Genetic analysis DNA sequencer:

The ABI PRISMa 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications.

The 310 Genetic Analyzer -- the ideal alternative to gel-based DNA analysis for any research, clinical, or forensic lab -- enables you to automate applications from de novo sequencing to SNP validation and linkage mapping. This versatile analyzer is so easy to use, that once it is installed and you have completed our short training course, all you have to do is load your samples, set up the Data Collection software and click the Start button.

 
 

ABI 310 DNA Sequencer

  • Sequencing Analysis Software
    Performs basecalling; assigns quality values; trims, displays, edits, and prints DNA sequencing data using the basecaller software.
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ABI span lang="en-us">Prism 3730xl DNA Analyzer

The ABI 3730xl DNA Analyzer, a 96-capillary sequencer, is the most robust sequencer on the market. With its production capacity of more than one million nucleotides per day, it is ideal for executing mega-large sequencing projects. It was brought to the market in 2002 by Applied Biosystems

The 3730xl Analyzer provides your laboratory
and production facility with very fast high quality genetic analysis and throughput. The high signal-to-noise ratio ensures you get high-quality data -- even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently. With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. Load and run up to 8 x 96 templates in continuous automated operation with less than 1 hour of instrument attendance/labor per 24 hour period.  In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

In addition to its 96 capillaries, the ABI 3730xl is equipped with an electrophoresis system, a laser, and a CCD camera. The capillaries, having a diameter of approximately 250 um, are filled with a polymer that acts as a molecular sieve. The DNA molecules are introduced into one end of the capillaries by electroinjection, and migrate through these capillaries under the attraction of high voltage (8500 V) at which point the molecules are separated according to their lengths. Close to the anode, a laser passes through each capillary and excites the fluorescent molecules incorporated onto the DNA during the reaction sequence. A CCD camera collects the information emitted by the fluorescent molecules as they pass in front of the laser. As each one of the four nucleic bases (A, C, G, and T) associates with a specific fluorochrome, the system distinguishes one base from another according to their respective wavelengths emitted. Subsequent data is analyzed, "base called," and filed when electrophoresis is complete.

We have multiple ABI units available for immediate shipment.

DESCRIPTION OF FUNCTIONS AND CAPABILITIES of the ABI 3700 sequencer:

  • Automated capillary gel eletrophoresis system

  • Run all four sequencing reactions in a single capillary

  • Run sequencing reactions based on dye-labeled terminator chemistry

  • Run three colors of microsatellites, or other fragments, and a size marker in a single lane

  • Utilize uncoated capillaries for higher reliability

  • Generate accurate sequence information with as little as 0.5 micrograms of template

  • Support fragment detection limit of less than 1.0 femtomole

  • Identify 500 bases of a -20M13 primed reaction

  • Detect 200 bases per hour per lane with 98.5% accuracy; detect over 500 bases at 98.5% accuracy at 100 bases/hour

  • Sheath flow technique detects labeled DNA in solution preventing signal loss and background noise caused by light scattering from capillary walls

  • Run 96 templates simultaneously with programable bypass system

  • Load and run up to 4 x 96 templates in continiuous automated operation with less than 1 hour of instrument attendance/labor per 24 hour period

  • Maintain active temperature control

  • Possess capability for different well to read distances

  • Both PC and Macintosh compatible

  • Support presentation of data on-screen or in printed format

  • Analyze data with baseline subtraction and scaling

  • Perform sequence analysis and fragment analysis

  • Provide data format for analysis as sequence, fragments, assembly into genes and genotypes

The capabilities to use multiple dyes to permit multiplexing or support dye-terminator chemistry are essential to the users of this instrument and to our productivity. 3700 is compatable with BigDye chemistry which is the state-of-the-art dye-labeled terminator chemistry.

These systems are used extensively by the genome centers, Celera, TIGR, etc. to sequence the human genome. The 3700 is a capillary electrophoresis system that can be used to sequence up to 96 DNA templates per run. The instrument accommodates up to four 96-well sample plates; a robotic arm loads the samples onto the capillaries; and electrophoresis is completed within five hours. Sample loading, electrophoresis, data collection, and data analysis have been automated to allow unattended operation. Because it is a capillary electrophoresis instrument, there is no need to manually pour or load gels. Benchmarking shows length of reads as long as 550 base pairs with 98% accuracy, depending on template and primer quality. The 3700 is an ideal platform for sequencing PCR products less than 550 base pairs in length. It also works well for single stranded and double stranded plasmid DNA templates. The length of read appears to be slightly less than that obtained on ABI 377 and 373 instruments.